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Other
Part:BBa_K4891018:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-09-19)
sgRNAldhA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids pBAD33-ldhA/adhE/poxB/pta and pTargetF-sgRNAldhA/adhE/poxB/pta serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
Source
ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.